Z2

Microorganism: Culture and single cell analysis

The main purpose of the Z2-project is the development of a platform that allows the isolation and identification of microorganisms associated with animal and plant metaorganisms that will be studied within the proposed CRC using standardized tools throughout all subprojects. Established methods and media for microbial cultivation as well as cutting edge tools for separating and analyzing single cells will be employed towards this goal. Overall we aim to achieve a high standardization of (i) isolation and identification of host-associated microorganisms, which are difficult to enrich and to grow in pure cultures, including development of new tools, and (ii) of hosts’ microbial community structure analysis by 16S rRNA sequencing approaches by coordinated protocols for DNA extraction, in order to guarantee comparable data sets of host associated consortia throughout all CRC projects. (iii) Furthermore, fluorescence activated cell sorting (FACS) will be employed as a tool to singularize individual microbial or eukaryotic cells from complex consortia and allow single cell analysis (e.g. single cell genomics) as well as cultivation of slow growing bacteria.

Researchers

Julia-Vanessa Böge

Technician
Kiel University Institute for General Microbiology GEOMAR - Helmholtz Centre for Ocean Research Kiel

Daniela Prasse

PhD Candidate
Kiel University Institute for General Microbiology

Publications

2017

Actinomycete Metabolome Induction/Suppression with N-Acetylglucosamine.

Dashti Y, Grkovic T, Abdelmohsen U R, Hentschel U, Quinn R J (2017); J Nat Prod., 80(4):828-836. doi: 10.1021/acs.jnatprod.6b00673

Draft Genome Sequences of “Candidatus Synechococcus spongiarum,” Cyanobacterial Symbionts of the Mediterranean Sponge Aplysina aerophoba.

Slaby B M, Hentschel U (2017); Genome Announc., 5(17):e00268-17. doi: 10.1128/genomeA.00268-17

Inhibitory potential of strepthonium A against Shiga toxin production in enterohemorrhagic Escherichia coli (EHEC) strain EDL933.

Cheng C, Balasubramanian S, Fekete A, Krischke M, Mueller M J, Hentschel U, Oelschlaeger T A, Abdelmohsen U R (2017); Nat Prod Res., 13:1-6. doi: 10.1080/14786419.2017.1297443

Construction and Screening of Marine Metagenomic Large Insert Libraries.

Weiland-Bräuer N, Langfeldt D, Schmitz R A (2017); Methods Mol Biol., 1539:23-42. doi: 10.1007/978-1-4939-6691-2_3

Marine Sponge-Derived Streptomyces sp. SBT343 Extract Inhibits Staphylococcal Biofilm Formation.

Balasubramanian S, Othman E M, Kampik D, Stopper H, Hentschel U, Ziebuhr W, Oelschlaeger T A, Abdelmohsen U R (2017); Front Microbiol., 8:236. doi: 10.3389/fmicb.2017.00236

Potential of marine natural products against drug-resistant fungal, viral, and parasitic infections.

Abdelmohsen U R, Balasubramanian S, Oelschlaeger T A, Grkovic T, Pham N B, Quinn R J, Hentschel U (2017); Lancet Infect Dis., 17(2):e30-e41. doi: 10.1016/S1473-3099(16)30323-1

2016

Emerging Sponge Models of Animal-Microbe Symbioses.

Pita L, Fraune S, Hentschel U (2016); Front Microbiol., 7:2102. doi: 10.3389/fmicb.2016.02102

Potential of marine natural products against drug-resistant fungal, viral, and parasitic infections.

Abdelmohsen U R, Balasubramanian S, Oelschlaeger T A, Grkovic T, Pham N B, Quinn R J, Hentschel U (2016); Lancet Infect Dis., pii:S1473-3099(16)30323-1. doi: 10.1016/S1473-3099(16)30323-1

Antifungal potential of marine natural products.

El-Hossary E M, Cheng C, Hamed M M, El-Sayed Hamed A N, Ohlsen K, Hentschel U, Abdelmohsen U R (2016);
Eur J Med Chem., 126:631-651. doi: 10.1016/j.ejmech.2016.11.022

An Enrichment of CRISPR and Other Defense-Related Features in Marine Sponge-Associated Microbial Metagenomes.

Horn H, Slaby B M, Jahn M T, Bayer K, Moitinho-Silva L, Förster F, Abdelmohsen U R, Hentschel U (2016); Front Microbiol., 7:1751. doi: 10.3389/fmicb.2016.01751

Shedding light on cell compartmentation in the candidate phylum Poribacteria by high resolution visualisation and transcriptional profiling.

Jahn M T, Markert S M, Ryu T, Ravasi T, Stigloher C, U Hentschel, Moitinho-Silva L (2016); Scientific Reports, 6:35860. doi: 10.1038/srep35860

Grapevine (Vitis vinifera) Crown Galls Host Distinct Microbiota.

Faist H, Keller A, Hentschel U, Deeken R (2016); Appl Environ Microbiol., 82(18):5542-52. doi: 10.1128/AEM.01131-16

Combination of bottom up 2D-LC-MS and semi-top-down GelFree-LC-MS enhances coverage of proteome and low molecular weight short open reading frame encoded peptides of the archaeon Methanosarcina mazei

Cassidy L , Prasse D, Linke D, Schmitz RA, Tholey A (2016); J Proteome Res. doi:10.1021/acs.jproteome.6b00569

Evaluation of 16S rRNA Gene Primer Pairs for Monitoring Microbial Community Structures Showed High Reproducibility within and Low Comparability between Datasets Generated with Multiple Archaeal and Bacterial Primer Pairs.

Fischer M A, Güllert S, Neulinger S C, Streit W R, Schmitz R A (2016); Front Microbiol., 7:1297. doi: 10.3389/fmicb.2016.01297

Widespread formation of alternative 3′ UTR isoforms via transcription termination in archaea

Dar D, Prasse D, Schmitz R A, Sorek R (2016); Nature Microbiology, 1(16143). doi: 10.1038/nmicrobiol.2016.143

Williamsia herbipolensis nov., isolated from the phyllosphere of Arabidopsis thaliana.

Kämpfer P, Busse H J, Horn H, Abdelmohsen U R, Hentschel U, Glaeser S P (2016); Int J Syst Evol Microbiol., doi: 10.1099/ijsem.0.001398

Highly effective inhibition of biofilm formation by the first 1 metagenome-derived AI-2 quenching enzyme.

Weiland-Bräuer N, Kisch M, Pinnow N, Liese A, Schmitz R A (2016); Front Microbiol., 7:1098. doi: 10.3389/fmicb.2016.01098

Antimicrobial and anti-Quorum Sensing activities of selected medicinal plants of Ethiopia: Implication for development of potent antimicrobial agents.

Bacha K, Tariku Y, Gebreyesus F, Zerihun S, Mohammed A, Weiland-Bräuer N, Schmitz R A, Mulat M (2016); BMC Microbiol., 16(1):139. doi: 10.1186/s12866-016-0765-9

Global Sponge Microbiome: Diversity, structure and convergent evolution of symbiont communities across the phylum Porifera.

Thomas T, Moitinho-Silva L, Lurgi M, Björk J R, Easson C, Astudillo C, Olson J B, Erwin P M, López-Legentil S, Luter H, C haves-Fonnegra A, Costa R, Schupp P, Steindler L, Erpenbeck D, Gilbert J, Knight R, Ackerman G, Lopez J V, Taylor M W, Thacker R , Montoya J M, Hentschel U, Webster N (2016); Nat Commun., 7:11870. doi: 10.1038/ncomms11870

Diversity, structure and convergent evolution of the global sponge microbiome.

Thomas T, Moitinho-Silva L, Lurgi M, Björk J R, Easson C, Astudillo-García C, Olson J B, Erwin P M, López-Legentil S, Luter H, Chaves-Fonnegra A, Costa R, Schupp P J, Steindler L, Erpenbeck D, Gilbert J, Knight R, Ackermann G, Victor Lopez J, Taylor M W, Thacker R W, Montoya J M, Hentschel U, Webster N S (2016); Nat Commun., 7:11870. doi: 10.1038/ncomms11870

Deep metagenome and metatranscriptome analyses of microbial communities affiliated with an industrial biogas fermenter, a cow rumen, and elephant feces reveal major differences in carbohydrate hydrolysis strategies.

Güllert S, Fischer M A, Turaev D, Noebauer B, Ilmberger N, Wemheuer B, Alawi M, Rattei T, Daniel R, Schmitz R A, Grundhoff A, Streit W R (2016); Biotechnology for Biofuels, 9(1):1-20. doi: 10.1186/s13068-016-0534-x

Quorum quenching–Stören friede zwischenbakterieller Beziehungen.

Schmitz-Streit R A, Weiland-Bräuer N (2016); BioSpektrum, 22(4):362-364. doi: 10.1007/s12268-016-0698-8

Restructuring of epibacterial communities on Fucus vesiculosus forma mytili in response to elevated pCO2 and increased temperature levels.

Mensch B, Neulinger S C, Graiff A, Pansch A, Künzel S, Fischer M A, Schmitz R A (2016); Frontiers in Microbiology. doi: 10.3389/fmicb.2016.00434

Methanosarcina flavescens sp. nov., a methanogenic archaeon isolated from a full-scale anaerobic digester.

Kern T, Fischer M A, Deppenmeier U, Schmitz R A, Rother M (2016); Int J Syst Evol Microbiol., 66:1533-1538.  doi: 10.1099/ijsem.0.000894

Hologenome analysis of two marine sponges with different microbiomes.

Ryu T, Seridi L, Moitinho-Silva L, Oates M, Liew Y J, Mavromatis C, Wang X, Haywood A, Lafi F F, Kupresanin M, Sougrat R, Alzahrani M A, Giles E, Ghosheh Y, Schunter C, Baumgarten S, Berumen M L, Gao X, Aranda M, Foret S, Gough J, Voolstra C R, Hentschel U, Ravasi T (2016); BMC Genomics., 17:158. doi: 10.1186/s12864-016-2501-0

Influence of mesoscale eddies on the distribution of nitrous oxide in the eastern tropical South Pacific.

Arévalo-Martínez D L, Kock A, Löscher C R, Schmitz R A, Stramma L, Bange H W (2016); Biogeosciences, 13:1105-1118. doi:10.5194/bg-13-1105-2016

Draft genome of the Arabidopsis thaliana phyllosphere bacterium, Williamsia sp. ARP1.

Horn H, Keller A, Hildebrandt U, Kämpfer P, Riederer M, Hentschel U (2016); Stand Genomic Sci., 11:8. doi: 10.1186/s40793-015-0122-x

2015

Draft genome sequences of three chemically rich actinomycetes isolated from Mediterranean sponges.

Horn H, Cheng C, Edrada-Ebel R, Hentschel U, Abdelmohsen U R (2015); Mar Genomics., 24(3):285-7. doi: 10.1016/j.margen.2015.10.003

Inhibitory activities of the marine streptomycete-derived compound SF2446A2 against Chlamydia trachomatis and Schistosoma mansoni.

Reimer A, Blohm A, Quack T, Grevelding C G, Kozjak-Pavlovic V, Rudel T, Hentschel U, Abdelmohsen U R (2015); J Antibiot (Tokyo)., 68(11):674-9. doi: 10.1038/ja.2015.54

Elicitation of secondary metabolism in actinomycetes.

Abdelmohsen U R, Grkovic T, Balasubramanian S, Kamel M S, Quinn R J, Hentschel U (2015); Biotechnol Adv., 33(6Pt1):798-811. doi: 10.1016/j.biotechadv.2015.06.003

First description of small proteins encoded by spRNAs in Methanosarcina mazei strain Gö1.

Prasse D, Thomsen J, De Santis R, Muntel J, Becher D, Schmitz R A (2015); Biochimie.; 117:138-48. doi: 10.1016/j.biochi.2015.04.007

Mining Genomes of Three Marine Sponge-Associated Actinobacterial Isolates for Secondary Metabolism.

Horn H, Hentschel U, Abdelmohsen U R (2015); Genome Announc., 3(5). pii:e01106-15. doi: 10.1128/genomeA.01106-15

Biodiversity, Anti-Trypanosomal Activity Screening, and Metabolomic Profiling of Actinomycetes Isolated from Mediterranean Sponges.

Cheng 1, MacIntyre L, Abdelmohsen U R, Horn H, Polymenakou P N, Edrada-Ebel R, Hentschel U (2015); PLoS One., 10(9):e0138528. doi: 10.1371/journal.pone.0138528

Composition of Bacterial Communities Associated with Aurelia aurita Changes with Compartment, Life Stage, and Population.

Weiland-Bräuer N, Neulinger S C, Pinnow N, Künzel S, Baines J F, Schmitz R A (2015); Appl Environ Microbiol., 81(17):6038-6052.  doi: 10.1128/AEM.01601-15

Dynamic changes of the luminal and mucosa-associated gut microbiota during and after antibiotic therapy with paromomycin.

Heinsen F A, Knecht H, Neulinger S C, Schmitz R A, Knecht C, Kühbacher T, Rosenstiel P C, Schreiber S, Friedrichs A K, Ott S J (2015); Gut Microbes., 6(4):243-54. doi: 10.1080/19490976.2015.1062959.

Lifestyle evolution in cyanobacterial symbionts of sponges.

Burgsdorf I, Slaby B M, Handley K M, Haber M, Blom J, Marshall C W, Gilbert J A, Hentschel U, Steindler L (2015); MBio., 6(3):e00391-15. doi: 10.1128/mBio.00391-15

Archaea associated with human surfaces: not to be underestimated.

Bang C, Schmitz R A (2015); FEMS Microbiology reviews., 39(5):631-48. doi: 10.1093/femsre/fuv010

Actinokineospora spheciospongiae sp. nov., isolated from the marine sponge Spheciospongia vagabunda.

Kämpfer P, Glaeser S P, Busse H J, Abdelmohsen U R, Ahmed S, Hentschel U (2015); Int J Syst Evol Microbiol., 65(Pt3):879-84. doi: 10.1099/ijs.0.000031

Antichlamydial sterol from the Red Sea sponge Callyspongia aff. implexa.

Abdelmohsen U R, Cheng C, Reimer A, Kozjak-Pavlovic V, Ibrahim A K, Rudel T, Hentschel U, Edrada-Ebel R, Ahmed S A (2015); Planta Med., 81(5):382-7. doi: 10.1055/s-0035-1545721
.

2014

Endophytes are hidden producers of maytansine in Putterlickia roots.

Kusari S, Lamshöft M, Kusari P, Gottfried S, Zühlke S, Louven K, Hentschel U, Kayser O, Spiteller M.(2014); J Nat Prod., 77(12):2577-84. doi: 10.1021/np500219a

GeoChip-based insights into the microbial functional gene repertoire of marine sponges (high microbial abundance, low microbial abundance) and seawater.

Bayer K, Moitinho-Silva L, Brümmer F, Cannistraci C V, Ravasi T, Hentschel U (2014); FEMS Microbiol Ecol., 90(3):832-43. doi: 10.1111/1574-6941.12441

Health- and disease-associated species clusters in complex natural biofilms determine the innate immune response in oral epithelial cells during biofilm maturation.

Langfeldt D, Neulinger S C, Stiesch M, Stumpp N, Bang C, Schmitz R A, Eberhard J (2014); FEMS Microbiology, 360(2):137-43. doi: 10.1111/1574-6968.12596

New antibacterial xanthone from the marine sponge-derived Micrococcus sp. EG45.

Eltamany E E, Abdelmohsen U R, Ibrahim A K, Hassanean H A, Hentschel U, Ahmed S A(2014); Bioorg Med Chem Lett., 24(21):4939-42. doi: 10.1016/j.bmcl.2014.09.040

Two new antioxidant actinosporin analogues from the calcium alginate beads culture of sponge-associated Actinokineospora sp. strain EG49.

Grkovic T, Abdelmohsen U R, Othman E M, Stopper H, Edrada-Ebel R, Hentschel U, Quinn R J (2014); Bioorg Med Chem Lett.,24(21):5089-92. doi: 10.1016/j.bmcl.2014.08.068

Facets of diazotrophy
in the oxygen minimum zone waters off Peru.

Löscher  C R, Großkopf T, Desai F D, Gill D, Schunck H, Croot P L , Schlosser C, 
Neulinger S C, Pinnow N, Lavik G, Kuypers M M M, LaRoche J, Schmitz R A (2014); ISME Journal, 8(11):2180-92. doi: 10.1038/ismej.2014.71

Revealing microbial functional
activities in the Red Sea sponge Stylissa carteri by metatranscriptomics.

Moitinho-Silva L, Seridi L, Ryu T, Voolstra C R, Ravasi T, Hentschel U (2014); Environ Microbiol, 16(12):3683–3698.  
doi: 10.1111/1462-2920.12533

Biofilm formation of mucosa-associated methanoarchaeal strains.

Bang C, Ehlers C, Orell A, Prasse D, Spinner M, Gorb S N, Albers S V, Schmitz R A (2014); 
Frontiers Microbiol., 5:353. doi: 10.3389/fmicb.2014.00353

The intestinal archaea Methanosphaera stadtmanae and Methanobrevibacter smithii activate human dendritic cells.

Bang C, Weidenbach K, Gutsmann T, Heine H, Schmitz R A (2014); PlosOne, 9(6):e99411. doi: 10.1371/journal.pone.0099411

Effects of β-lactam antibiotics and fluoroquinolones on human gut microbiota in relation to Clostridium difficile associated diarrhea.

Knecht H, Neulinger S C, Heinsen F A, Knecht C, Schilhabel A, Schmitz R A, Zimmermann A, dos Santos V M, Ferrer M, Rosenstiel P C, Schreiber S, Friedrichs A K, Ott S J (2014); PLoS One., 9(2):e89417. doi: 10.1371/journal.pone.0089417

Thioredoxin targets fundamental processes in a methane-producing
 archaeon, Methanocaldococcus jannaschii.

Susanti D, Wong J H, Vensel W H, Loganathan U, DeSantis R, Schmitz R A, Balsera M, Buchanan B B, Mukhopadhyay B (2014); Proc Natl Acad Sci USA, 111(7):2608-13. doi: 10.1073/pnas.1324240111

An environmental
 bacterial taxon with a large and distinct metabolic repertoire.

Wilson M C, Mori T, Rückert C, Uria A R, Helf M J, Takada K, Gernert C, Steffens U A, Heycke N, 
Schmitt S, Rinke C, Helfrich E J, Brachmann A O, Gurgui C, Wakimoto T, Kracht M, Crüsemann M,
 Hentschel U, Abe I, Matsunaga S, Kalinowski J, Takeyama H, Piel J (2014); Nature, 506:58-62. doi: 10.1038/nature12959

Composition of microbial supragingival biofilms in young healthy adults in correlation to experimentally induced gingivitis.

Langfeldt D, Neulinger S C, Heuer W, Staufenbiel I, Sven Künzel S, Baines J F, Eberhard J, Schmitz R A (2014); PLosOne, 9(2):e87449. doi: 10.1371/journal.pone.0087449

Small regulatory RNAs in Archaea.

Babski J, Maier L K, Heyer R, Jaschinski K, Prasse D, Jäger D, Randau L, Schmitz R A, Marchfelder A, Soppa J (2014); RNA Biol., 11(5):484-93. doi: 10.4161/rna.28452

2013

Single-cell genomics reveals complex carbohydrate degradation patterns in poribacterial
symbionts of marine sponges.

Kamke J, Sczyrba A, Ivanova N, Schwientek P, Rinke C, Mavromatis K, Woyke T, Hentschel U
 (2013); ISME J, 7(12):2287-2300. doi: 10.1038/ismej.2013.111

Animals in a bacterial world, a new imperative for the life sciences.

McFall-Ngai M, Hadfield M, Bosch T C G, Carey H, Domazet-Loso T, Douglas A, Dubilier N, Eberl G, Fukami T, Gilbert S, Hentschel U, King N, Kjelleberg S, Knoll A, Kremer N, Mazmanian S, Metcalf J, Nealson K, Pierce N, Rawls J, Reid A, Ruby E, Rumpho M, Sanders J, Tautz D, Wernegreen J (2013); Proc Natl Acad Sci USA, 110(9):3229-36. doi: 10.1073/pnas.1218525110

Low-level inflammation in the prevalent oral disease gingivitis induces surrogate markers of atherosclerosis in young healthy individuals. An interventional study.

Eberhard J , Grote K, Luchtefeld M, Heuer W, Schuett H, Divchev D, Scherer R, Schmitz R A, Langfeldt D, Stumpp N, Staufenbiel I, Schieffer B, Stiesch M (2013); PLOS one, 8(2):e55265.  doi: 10.1371/journal.pone.0055265

Regulatory RNAs in archaea: first target identification in Methanoarchaea.

Prasse D, Ehlers C, Backofen R, Schmitz R A (2013); Biochem Soc Trans., 41(1):344-9. doi: 10.1042/BST20120280

2012

An archaeal
sRNA targeting cis- and trans-encoded mRNAs via two distinct domains.

Jäger D, Pernitzsch S R, Richter A, Backofen R, Sharma C M, Schmitz R A (2012); Nucleic Acids
Res., 40:10964-79. doi: 10.1093/nar/gks847

N2 fixation rates in the Atlantic Ocean have been severely underestimated.

Großkopf, T, W. Mohr, T. Baustian, H. Schunck, D. Gill, M.M.M. Kuypers, G. Lavik, R.A. Schmitz, 
D.W.R. Wallace, J. LaRoche (2012); 
Nature, 488(7411):361-4.

Assessing the complex sponge microbiota – core,
variable, and species-specific bacterial communities in marine sponges.

Schmitt S, Tsai P, Bell J, Fromont F, Ilan M, Lindquist N L, Perez T, Rodrigo A, Schupp P J, Vacelet 
J, Webster N, Hentschel U, Taylor M W (2012); ISME J, 6(3): 564-57. doi: 10.1038/ismej.2011.116

Genomic insights into the marine sponge microbiome.

Hentschel U, Piel J, Degnan S M, Taylor M W (2012);
 Nat Rev Microbiol., 10(9): 641-654. doi: 10.1038/nrmicro2839

Small RNAs for defence and regulation in archaea.

Marchfelder A, Fischer S, Brendel J, Stoll B, Maier L K, Jäger D, Prasse D, Plagens A, Schmitz R A, Randau L (2012); Extremophiles., 16(5):685-96. doi: 10.1007/s00792-012-0469-5

Effects of Antimicrobial Peptides on Methanogenic Archaea.

Bang C, Schilhabel A, Weidenbach K, Kopp A, Goldmann T, Gutsmann T, Schmitz R A (2012); Antimicrobial Agents and Chemotherapy, 56(8):4123-30. doi:

2011

rRNA-based profiling of bacteria in the axilla of healthy males suggests right-left asymmetry in bacterial activity.

Egert M, Schmidt I, Höhne H-M, Lachnit T, Schmitz R , Breves R (2011); FEMS Microbiology Ecology, 77:146-153. doi: 10.1111/j.1574-6941.2011.01097.x

Epibacterial community patterns on marine macroalgae are host-specific but temporally variable.

Lachnit T, Meske D, Wahl M, Harder T, Schmitz R A (2011); Environmental Microbiology, 13(3):655-65. doi: 10.1111/j.1462-2920.2010.02371.x

Single
-cell genomics reveals the lifestyle of Poribacteria, a candidate phylum symbiotically associated
with marine sponges.

Siegl A, Kamke J, Hochmuth T, Piel J, Richter M, Liang C, Dandekar T, Hentschel U (2011); ISME J, 5(1):61-70. doi: 10.1038/ismej.2010.95

2010

Construction and screening of marine metagenomic libraries.

Weiland N, Löscher C, Metzger R, Schmitz R (2010); Methods Mol Biol., 668:51-65. doi: 10.1007/978-1-60761-823-2_3

2009

Deep sequencing
analysis of the Methanosarcina mazei Gö1 transcriptome in response to nitrogen availability.

Jäger D, Sharma C M, Thomsen J, Ehlers C, Vogel J, Schmitz R A (2009); Proc.
Nat. Acad. Sci. USA, 106(51):21878-21882. doi: 10.1073/pnas.0909051106

2008

Molecular microbial diversity
survey of sponge reproductive stages and mechanistic insights into vertical transmission of microbial
symbionts.

Schmitt S, Angermeier H, Schiller R, Lindquist N, Hentschel U (2008); Appl Environ Microbiol, 74:7694-7708. doi: 10.1128/AEM.00878-08

Physiology, phylogeny and in-situ evidence for bacterial
and archaeal nitrifiers in the marine sponge Aplysina aerophoba.

Bayer K, Schmitt S, Hentschel U (2008); Environ Microbiol, 10(11):2942-
2955. doi: 10.1111/j.1462-2920.2008.01582.x

2005

Unique mechanistic
features of post translational regulation of glutamine synthetase activity in Methanosarcina mazei in
response to nitrogen availability.

Ehlers C, Weidenbach K, Veit K, Forchhammer K and Schmitz R A (2005); Mol. Microbiol., 55:1841-1854. doi: 10.1111/j.1365-2958.2005.04511.x

2004

Metagenomics – the key to the uncultured microbes.

Streit W R, Schmitz R A (2004); Opin. Microbiol., 7:492–498. doi: 10.1016/j.mib.2004.08.002

Discovery of a novel candidate phylum ‘Poribacteria’
in marine sponges.

Fieseler L, Horn M, Wagner M, Hentschel U (2004); Appl Environ Microbiol, 70(6):3724-3732. doi: 10.1128/AEM.70.6.3724-3732.2004

2000

Screening of environmental 
DNA libraries for the presence of genes conferring lipolytic activity to Escherichia coli.

Henne A, Schmitz R A, Bömeke M, Gottschalk G, Daniel R (2000); Appl. Environ.
 Microbiol., 66:3113-3116. doi: 10.1128/AEM.66.7.3113-3116.2000