The main purpose of the Z2 project is to maintain and continue the CRC platform that allows for the isolation and identification of prokaryotic microorganisms and / or consortia associated with animal and plant metaorganisms studied within the second funding phase of the CRC. Established methods and media for microbial enrichment and cultivation as well as cutting edge novel tools for separating and analyzing single cells will be employed towards this goal. Overall we aim to achieve
(i) isolation and identification of host-associated microorganisms, which are difficult to enrich and to grow in pure cultures, including development of new tools,
(ii) enrich and isolate microbial (synthrophic) consortia,
(iii) establish and supply the different CRC projects with simplified stand-ardized Mock cultures for recolonization experiments of germ-free hosts.