Next generation sequencing applications for studying metaorganisms

The main purpose of the Z3-project is to provide a standardized central hub for next generation sequencing(NGS)-based applications among CRC 1182 projects. This includes the metagenomic analysis of host-associated microbial communities, bacterial genome sequencing and the individual transcriptomic analysis of both hosts and microbes.

The metagenomic sequencing will be provided on two levels:
(i) bacterial community profiling based on 16S rRNA gene amplicon sequencing and
(ii) functional (shotgun) metagenomics.

Transcriptomic analyses will be provided using standardized RNAseq protocols using different RNA purification methods. The technical sequence assessment will be performed using the NGS infrastructure at the Institute of Clinical Molecular Biology.

The main goal of the project is to maintain a high level of standardization to ensure comparability between the diverse data sets generated by the CRC 1182. Further, the Z3 project will closely monitor the most current technical advances in the field and provide these to the CRC 1182, when possible.


Jan Schubert

Kiel University Institute for Experimental Medicine

Prof. Dr. John Baines

Principal Investigator
Kiel University Institute for Experimental Medicine Max-Planck-Institute for Evolutionary Biology



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