The primary task of the Z3 project is to provide a specialized platform for next generation sequencing-based and proteomics-based applications to the individual CRC1182 subprojects, tailored for specific requirements when studying the origin and function of metaorganisms.
Next Generation Sequencing: The Z3 is equipped with an established infrastructure and workflow to provide a large portfolio of sequencing-based applications, covering the host side – independent of species or kingdom – as well as the microbial side. This includes whole genome sequencing, especially de novo whole genome sequencing, whole exome sequencing, epigenome analysis via reduced representation bisulfide sequencing, transcriptome analysis as well as bacterial profiling via 16S rRNA gene amplicon sequencing and functional shotgun metagenomics/metatranscriptomics. Adjusting the sample and data processing architecture to the needs of the CRC 1182, which is supported by the in-house Comprehensive Centre for Genome Analysis (CCGA), provides a maximum of standardization and reproducibility to the CRC members, while maintaining the flexibility that is required to implement the most recent technological developments.
Proteomics: Liquid chromatography-mass spectrometry (LC-MS) based proteomics, peptidomics and metabolite analytics will provide analytical technologies to enable the deciphering of molecular mechanisms triggering the interaction of organisms as well as the reaction of metaorganisms on biotic and abiotic environmental factors. Technologies available encompass methods for the identification, quantification and molecular characterization (posttranslational modifications) of peptides and proteins/proteomes as well as methods for MS-based imaging and isotope labeling to follow exchange of molecules between organisms and for the elucidation of enzymatic activities controlling the inter-organism interactions.