B2
Recognition and Communication

Microbiota-host interactions at the base of the metazoan tree

The overarching aims addressed within the B-projects on “Recognition & Communication” are to elucidate mechanisms responsible for shaping microbial communities and compare the different functions and consequences of microorganisms for the respective metaorganisms. Studying the cnidarian Aurelia aurita, which undergoes a complex life cycle, we demonstrated that in the absence of its natural microbiome, fitness and asexual reproduction are severely compromised. This crucial microbial impact on metamorphosis towards regular offspring generation is pivotal at the polyp stage. Moreover, we obtained strong indications that the host A. aurita, as well as Mnemiopsis leidyi, uses Quorum Quenching (QQ) as a host defense mechanism to control bacterial colonization.

The overall aims of this project are to gain insights into the molecular mechanisms of respective microbe-host interactions and address the hypothesis that host-derived QQ activities shape the associated microbiota. Specifically, we now aim in B2.1 to:

(i) Identify the required microbiota’s function for a regular life cycle of A. aurita (regular strobilation, offspring generation) by using artificially reduced microbiota as well as genetically engineered microbes in recolonization experiments.

(ii) Decipher the role of host-derived QQ activities in host-microbe interactions. Analyzing expression patterns of already identified QQ-ORFs of A. aurita when challenged with foreign versus naturally occurring bacteria, as well as opportunistic pathogens, combined with biochemistry and genetic approaches, will allow us to gain insights into A. aurita’s immune response and particularly on host-derived QQ.

    In addition, a comparative analysis with three other invertebrate hosts in close collaboration with the B1 project will deepen our understanding of innate invertebrate immune responses and particularly elucidate the impact of QQ as an additional, fundamental defense strategy. The latter has the potential to be a fascinating finding, enlarging the host’s repertoire of mechanisms to shape its microbiome.

    Overall, focusing on mechanisms of host-bacterial interactions and host recognition of native vs. non-native microbes, we expect to reveal specific mechanisms and general principles that can be used to better understand and manipulate community composition within the metaorganism.

    B2
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    Researchers

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    B2.1: Functional repertoire of A. aurita's microbiota crucial for asexual reproduction and the role of host-derived Quorum Quenching for host microbe interaction

    B2.2 (E): The role of associated microbiota for fitness and invasion success in the comb jelly Mnemiopsis leidyi

    B2
    Related Publications

    Related Publications

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    Year All
    2023
    B2

    Four novel Caudoviricetes bacteriophages isolated from Baltic Sea water infect colonizers of Aurelia aurita

    Stante M, Weiland-Bräuer N, Repnik U, Werner A, Bramkamp M, Chibani CM, Schmitz RA (2023) Four novel Caudoviricetes bacteriophages isolated from Baltic Sea water infect colonizers of Aurelia aurita. Viruses. 2023; 15(7):1525. doi: 10.3390/v1507152
    2023
    A2
    B2
    B4

    Comparative genomics of novel Bacteroides acidifaciens isolates reveals candidates for adaptation to host subspecies in house mice

    Hanna Fokt, Shauni Doms, Malte C. Rühlemann, Maxime Godfroid, Ruth A. Schmitz, Britt M. Hermes, John F. Baines (2023) Comparative genomics of novel Bacteroides acidifaciens isolates reveals candidates for adaptation to host subspecies in house mice BioRxiv

     

    2023
    B2

    Microbial community changes correlate with impaired host fitness of Aurelia aurita after environmental challenge

    Pinnow N, Chibani CM, Güllert S, Weiland-Bräuer N (2023) Microbial community changes correlate with impaired host fitness of Aurelia aurita after environmental challenge. anim microbiome 5, 45 (2023). doi: 10.1186/s42523-023-00266-4

    2023
    B2

    Antimicrobial peptides originating from expression libraries of Aurelia aurita and Mnemiopsis leidyi prevent biofilm formation of opportunistic pathogens

    Ladewig L, Gloy L, Langfeldt D, Pinnow N, Weiland-Bräuer N, Schmitz RA (2023) Antimicrobial peptides originating from expression libraries of Aurelia aurita and Mnemiopsis leidyi prevent biofilm formation of opportunistic pathogens. Microorganisms, 11(9), 2184. doi: 10.3390/microorganisms11092184

    2023
    B2

    First insights into the Aurelia aurita transcriptome response upon manipulation of its microbiome

    Weiland-Bräuer N, Koutsouveli V, Langfeldt D, Schmitz RA (2023) First insights into the Aurelia aurita transcriptome response upon manipulation of its microbiome. Front. Microbiol. 14:1183627. doi: 10.3389/fmicb.2023.1183627

    2023
    B2

    The Life Cycle of Aurelia aurita Depends on the Presence of a Microbiome in Polyps Prior to Onset of Strobilation

    Jensen N, Weiland-Bräuer N, Joel S, Chibani CM, Schmitz RA (2023)  The Life Cycle of Aurelia aurita Depends on the Presence of a Microbiome in Polyps Prior to Onset of Strobilation. Microbiology Spectrum, 11(4), e00262-23. doi: 10.1128/spectrum.00262-23

    2023
    A1
    A2
    B2
    B4
    Z2
    Z3

    Closely related Bacteroides of the murine intestinal microbiota affect each other’s growth positively or negatively

    Hanna Fokt, Gabija Sakalyte, Rahul Unni, Mohammad Abukhalaf, Liam Cassidy, Georgios Marinos, Maxime Godfroid, Birhanu M Kinfu, Ruth A Schmitz, Christoph Kaleta, Andreas Tholey, John F Baines, Tal Dagan, Daniel Unterweger (2023) BioRxiv
    2021
    B2

    Invasion genomics uncover contrasting scenarios of genetic diversity in a widespread marine invader.

    Jaspers C, Ehrlich M, Pujolar JM, Künzel S, Bayer T, Limborg MT, Lombard F, Browne WE, Stefanova K, Reusch TBH (2021):  PNAS. 118(51)e2116211118. doi: 10.1073/pnas.2116211118

    2020
    B2

    The native microbiome is crucial for offspring generation and fitness of Aurelia aurita

    Weiland-Bräuer N, Pinnow N, Langfeldt D, Roik A, Güllert S, Chibani CM, Reusch TBH, Schmitz RA (2020) mBio. 11(6) e02336-20. doi: 10.1128/mBio.02336-20

    2020
    B2

    B-vitamin nutrition in the pea aphid-Buchnera symbiosis

    Blow F, Bueno E, Clark N, Zhu TD, Chung SH, Güllert S, Schmitz RA, Douglas AE (2020) Journal of Insect Physiology. 126:104092. doi: 10.1016/j.jinsphys.2020.104092

    2020
    B2
    Z2

    Cultivable microbiota associated with Aurelia aurita and Mnemiopsis leidyi

    Weiland-Bräuer N, Prasse D, Brauer A, Jaspers C, Reusch TBH, Schmitz RA (2020) MicrobiologyOpen. 9(9):e1094 doi: 10.1002/mbo3.1094  

    2020
    A2
    B2

    Differences in the microbiota of native and non-indigenous gelatinous zooplankton organisms in a low saline environment

    Jaspers C, Weiland-Bräuer N, Rühlemann MC, Baines JF, Schmitz RA, Reusch TBH (2020) Sci Total Environ. 734:139471. doi: 10.1016/j.scitotenv.2020.139471

    2019
    A3
    A4
    B2
    C1
    C2
    INF

    Advancing our functional understanding of host–microbiota interactions: a need for new types of studies

    He J, Lange J, Marinos G, Bathia J, Harris D, Soluch R, Vaibhvi V, Deines P, Hassani MA, Wagner K-S, Zapien‐Campos R, Jaspers C, Sommer F (2019) BioEssays, 1900211 (1-5). doi: 10.1002/bies.201900211

     

    2019
    B2
    INF
    Z3

    Host-microbe interactions in the chemosynthetic Riftia pachyptila symbiosis

    Hinzke T, Kleiner M, Breusing C, Felbeck H, Häsler R, Sievert SM, Schlüter R, Rosenstiel P, Reusch TBH, Schweder T, Markert S (2019) mBio 10:6, e02243-19. doi: 10.1128/mBio.02243-19

    2019
    B2

    Microbiota differences of the comb jelly Mnemiopsis leidyi in native and invasive sub-populations

    Jaspers C, Weiland-Bräuer N, Fischer M, Künzel S, Schmitz RA, Reusch TBH (2019) Front. Mar. Sci. 6: 635. doi: 10.3389/fmars.2019.00635

    2019
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    INF
    Z3

    Comparative analysis of amplicon and metagenomic sequencing methods reveals key features in the evolution of animal metaorganisms

    Rausch P, Rühlemann M, Hermes BM, Doms S, Dagan T, Dierking K, Domin H, Fraune S, von Frieling J, Hentschel U, Heinsen F-A, Höppner M, Jahn MT, Jaspers C, Kissoyan KAB, Langfeldt D, Rehman A, Reusch TBH, Roeder T, Schmitz RA, Schulenburg H, Soluch R, Sommer F, Stukenbrock E, Weiland-Bräuer N, Rosenstiel P, Franke A, Bosch T, Baines JF (2019) Microbiome, doi: 10.1186/s40168-019-0743-1

    2019
    A2
    A4
    B1
    B2

    Neutrality in the metaorganism

    Sieber M, Pita L, Weiland-Bräuer N, Dirksen P, Wang J, Mortzfeld B, Franzenburg S, Schmitz RA, Baines JF, Fraune S, Hentschel U, Schulenburg H, Bosch TCG, Traulsen A (2019) PLoS Biol., DOI: 10.1371/journal.pbio.3000298

    2019
    A4
    B1
    B2

    Resolving structure and function of metaorganisms through a holistic framework 2 combining reductionist and integrative approaches

    Jaspers C, Fraune S, Consortium of Australian Academy of Science Boden Research Conference Participants, Arnold AE, Miller DJ, Bosch TCG, Voolstra CR (2019) Zoology. doi: 10.1016/j.zool.2019.02.007

    2019
    B2

    Potential role of host-derived quorum quenching in modulating bacterial colonization in the moon jellyfish Aurelia aurita

    Weiland-Bräuer N, Pinnow N, Fischer MA, Schmitz RA (2019) Scientific Reports, 9(1), 34. DOI:10.1038/s41598-018-37321-z

    2018
    A2
    B2
    C1
    C2
    INF
    Z2

    Functions of the Microbiota for the Physiology of Animal Metaorganisms

    Esser D, · Lange J, · Marinos G, · Sieber M, Best L, Prasse D, Bathia J, Rühlemann MC, Boersch K, Jaspers C, Sommer F (2018) J Innate Immun DOI: 10.1159/000495115

    2018
    A1
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    B1
    B2
    C1
    Z1

    Metaorganisms in extreme environments: do microbes play a role in organismal adaptation?

    Bang C, Dagan T, Deines P, Dubilier N, Duschl W J, Fraune S, Hentschel U, Hirt H, Hülter N, Lachnit T, Picazo D, Galan P L, Pogoreutz C, Rädecker N, Saad M M, Schmitz R A, Schulenburg H, Voolstra C R, Weiland-Bräuer N, Ziegler M, Bosch T C G (2018); Zoology, doi: 10.1016/j.zool.2018.02.004

    2017
    B2

    Microbial contributions to the persistence of coral reefs.

    Webster N S, Reusch T B H (2017); ISME J., doi: 10.1038/ismej.2017.66

    2016
    A1
    B2

    Combination of Bottom-up 2D-LC-MS and Semi-top-down GelFree-LC-MS Enhances Coverage of Proteome and Low Molecular Weight Short Open Reading Frame Encoded Peptides of the Archaeon Methanosarcina mazei.

    Cassidy L, Prasse D, Linke D, Schmitz R A, Tholey A (2016)
    J Proteome Res., 15(10):3773-3783. doi: 10.1021/acs.jproteome.6b00569

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