Principal Investigator

Prof. Dr. Andreas Tholey

Institution

Kiel University
University Hospital Schleswig Holstein
Institute for Experimental Medicine
Systematic Proteomics and Bioanalytics Lab
Vita

Education/Training

2007
Habilitation for Analytical Biochemistry, Saarland University

1999
Dr. rer.nat, Saarland University / DKFZ Heidelberg

1995
Dipl. Chem., Saarland University

1989 – 1995
Study of Chemistry, Saarland University, Saarbrücken

Research Experience/Academic Appointments

Since 2008
Group leader and W2-professor for Systematic Proteome Research & Bioanalytics at the Christian-Albrechts-University of Kiel (position originally funded via DFG-Cluster of Excellence “Inflammation at Interfaces”; now: permanent position)

2010
Guest-professorship at Université de Pau, France

2004
Research Stage at the “Laboratoire de Spectrometrie de masse Bio-Organique”, University of Strasbourg/ CNRS, Strasbourg, France

1999 – 2007
Postdoctoral fellow and Habilitation (C1), Saarland University

1995 – 1998
Scientific assistant, German Cancer Research Centre (DKFZ), Heidelberg

Important Scientific Prizes/Functions

Since 2012
Member of the Steering-Committee of the DFG-Cluster of Excellence-308 “Inflammation at Interfaces”

Since 2011
Vice-chair of initiative on multi-organism proteomics (iMOP) within human proteome organization (HUPO)

Contact

+49 431 500 30300
+49 431 500 30308
a.tholey@iem.uni-kiel.de
https://www.iem.uni-kiel.de/de/systematische-proteomics/mitarbeiter https://gepris.dfg.de/gepris/person/1794581

Associated Research Groups

Prof. Dr. Andreas Tholey
Publications

Publications

Filter by

Year All
2023
A2
C2
Z3

A mitochondrial sirtuin shapes the intestinal microbiota by controlling lysozyme expression

Knop M, Treitz C, Bettendorf S, Bossen J, von Frieling J, Doms S, Bruchhaus I, Kuehnlein RP, Baines JF, Tholey A, Roeder T (2023) A mitochondrial sirtuin shapes the intestinal microbiota by controlling lysozyme expression. bioRxiv, doi: https://doi.org/10.1101/2023.06.02.543385

2023
Z3

Digital microfluidics and magnetic bead-based intact proteoform elution for quantitative top-down nanoproteomics of single C. elegans nematodes

Leipert J, Kaulich PT, Steinbach MK, Steer B, Winkels K, Blurton C, Leippe M, Tholey A (2023). Digital microfluidics and magnetic bead-based intact proteoform elution for quantitative top-down nanoproteomics of single C. elegans nematodes. Angew Chem, e202301969. Doi: 10.1002/anie.202301969

2023
A4
Z3

Bacterial c-di-GMP has a key role in establishing host-microbe symbiosis

Obeng N, Czerwinski A, Schütz D, Michels J, Leipert J, Bansept F, García García MJ, Schultheiß T, Kemlein M, Fuß J, Tholey A, Traulsen A, Sondermann H, Schulenburg H (2023) Bacterial c-di-GMP has a key role in establishing host-microbe symbiosis. Nature Microbiology doi: 10.1038/s41564-023-01468-x.

2023
A1
A4
Z3

The C. elegans proteome response to two protective Pseudomonas symbionts

Barbara Pees*, Lena Peters, Christian Treitz, Inga K. Hamerich, Kohar A. B. Kissoyan, Andreas Tholey, Katja Dierking* (2023) The C. elegans proteome response to two protective Pseudomonas symbionts. bioRxiv doi:10.1101/2023.03.22.533766. *Shared corresponding authors.

2023
A1
A2
B2
B4
Z2
Z3

Closely related Bacteroides of the murine intestinal microbiota affect each other’s growth positively or negatively

Hanna Fokt, Gabija Sakalyte, Rahul Unni, Mohammad Abukhalaf, Liam Cassidy, Georgios Marinos, Maxime Godfroid, Birhanu M Kinfu, Ruth A Schmitz, Christoph Kaleta, Andreas Tholey, John F Baines, Tal Dagan, Daniel Unterweger (2023) BioRxiv doi: https://doi.org/10.1101/2023.03.05.530569
2023
A2
C2
Z3

A mitochondrial sirtuin shapes the intestinal microbiota by controlling lysozyme expression

Knop M, Treitz C, Bettendorf S, Bossen J, von Frieling J, Doms S, Bruchhaus I, Kuehnlein RP, Baines JF, Tholey A, Roeder T (2023) A mitochondrial sirtuin shapes the intestinal microbiota by controlling lysozyme expression. bioRxiv, doi: https://doi.org/10.1101/2023.06.02.543385

2022
Z3

Digital microfluidics supported microproteomics for quantitative proteome analysis of single Caenorhabditis elegans nematodes

Steinbach M, Leipert J, Blurton C, Leippe M, Tholey A (2022). Digital microfluidics supported microproteomics for quantitative proteome analysis of single Caenorhabditis elegans nematodes. J Proteome Res, 21: 1986-1996. doi: 10.1021/acs.jproteome.2c00274.

2021
Z3

Isobaric peptide labeling on digital microfluidics for quantitative low cell number proteomics.

Leipert J, Steinbach MK, Tholey A (2021) Anal Chem. 93(15):6278-6286. doi: 10.1021/acs.analchem.1c01205.

2019
A1
C2
Featured

Prdx4 limits caspase-1 activation and restricts inflammasome-mediated signaling by extracellular vesicles

Lipinski S, Pfeuffer S, Arnold P, Treitz C, Aden K, Ebsen H, Falk-Paulsen M, Gisch N, Fazio A, Kuiper J, Luzius A, Billmann-Born S, Schreiber S, Nuñez G, Beer HD, Strowig T, Lamkanfi M, Tholey A, Rosenstiel P (2019) EMBO J. 38(20) doi: 10.15252/embj.2018101266.

2019
A1
A4
INF
Z3

The inducible response of the nematode Caenorhabditis elegans to members of its natural microbiome across development and adult life

Yang W#, Petersen C#, Pees B#, Zimmermann J, Waschina S, Dirksen P, Rosenstiel P, Tholey A, Leippe M, Dierking K, Kaleta C*, Schulenburg H*.  Front Microbiol. 10:1793. # Equal contribution as first authors, * Equal contribution as senior authors doi: 10.3389/fmicb.2019.01793.

2018
A1

The Caenorhabditis elegans proteome response to naturally associated microbiome members of the genus Ochrobactrum

Cassidy L, Petersen C, Treitz C, Dierking K, Schulenburg H, Leippe M, Tholey A (2018); Proteomics, doi: 10.1002/pmic.201700426

2018
A1

Miniaturized dispersive liquid-liquid microextraction and MALDI MS using ionic liquid matrices for the detection of bacterial communication molecules and virulence factors.

Leipert J, Bobis I, Schubert S, Fickenscher H, Leippe M, Tholey A (2018); Anal Bioanal Chem. , pp 1–12. doi: 10.1007/s00216-018-0937-6

2017
A1

We Are Not Alone: The iMOP Initiative and Its Roles in a Biology- and Disease-Driven Human Proteome Project.

Tholey A, Taylor N L, Heazlewood J L, Bendixen E (2017); J Proteome Res., 16(12):4273-4280. doi: 10.1021/acs.jproteome.7b00408

2017
A1

Insights into Microalga and Bacteria Interactions of Selected Phycosphere Biofilms Using Metagenomic, Transcriptomic, and Proteomic Approaches.

Krohn-Molt I, Alawi M, Förstner K U, Wiegandt A, Burkhardt L, Indenbirken D, Thieß M, Grundhoff A, Kehr J, Tholey A, Streit W R (2017); Front Microbiol., doi: 10.3389/fmicb.2017.01941

2017
A1

Identification and Quantification of N-Acyl Homoserine Lactones Involved in Bacterial Communication by Small-Scale Synthesis of Internal Standards and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.

Leipert J, Treitz C, Leippe M, Tholey A (2017); J Am Soc Mass Spectrom., 28(12):2538-2547. doi: 10.1007/s13361-017-1777-x

2017
A1
C2
Z3
Featured

Efficacy of Sterile Fecal Filtrate Transfer for Treating Patients With Clostridium difficile Infection. Gastroenterology.

Ott S J, Waetzig G H, Rehman A, Moltzau-Anderson J, Bharti R, Grasis J A, Cassidy L, Tholey A, Fickenscher H, Seegert D, Rosenstiel P, Schreiber S (2017); Gastroenterology, 152(4):799-811.e7. doi: 10.1053/j.gastro.2016.11.010

2016
A1

Differential quantitative proteome analysis of Escherichia coli grown on acetate versus glucose.

Treitz C, Enjalbert B, Portais J C, Letisse F, Tholey A (2016); Proteomics., 16(21):2742-2746. doi: 10.1002/pmic.201600303

2016
A1
B2

Combination of Bottom-up 2D-LC-MS and Semi-top-down GelFree-LC-MS Enhances Coverage of Proteome and Low Molecular Weight Short Open Reading Frame Encoded Peptides of the Archaeon Methanosarcina mazei.

Cassidy L, Prasse D, Linke D, Schmitz R A, Tholey A (2016)
J Proteome Res., 15(10):3773-3783. doi: 10.1021/acs.jproteome.6b00569

Institutions & Partners